Journal: Annals of Translational Medicine

Article Title: Identifying potential therapeutic targets of Tang-Yi-Ping for the treatment of impaired glucose tolerance: a tandem mass tag-labeled quantitative proteomic analysis

doi: 10.21037/atm-21-4257

Figure Lengend Snippet: Specific information on 16 protein targets in the treatment of IGT with TYP

Article Snippet: For example, the expression levels of Rbp4 (boster, #PB0368, China) and Flot2 (boster, #PB0663, China) in the 3 groups were calculated with GAPDH (boster, #BM1623, China) as an internal reference.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Identifying potential therapeutic targets of Tang-Yi-Ping for the treatment of impaired glucose tolerance: a tandem mass tag-labeled quantitative proteomic analysis

doi: 10.21037/atm-21-4257

Figure Lengend Snippet: Relative expression levels of Rbp4 and Flot2 proteins in pancreatic samples of the 3 groups. Rbp4: *, P<0.05, vs. Control; #, P<0.05, vs. IGT. Flot: *, P<0.05, vs. Control; #, P<0.05, vs. IGT. IGT, impaired glucose tolerance.

Article Snippet: For example, the expression levels of Rbp4 (boster, #PB0368, China) and Flot2 (boster, #PB0663, China) in the 3 groups were calculated with GAPDH (boster, #BM1623, China) as an internal reference.

Techniques: Expressing, Control

Journal: Autophagy

Article Title: Astrocytes promote progression of breast cancer metastases to the brain via a KISS1-mediated autophagy

doi: 10.1080/15548627.2017.1360466

Figure Lengend Snippet: Detection of CSC in the primary BrCa specimens and brain metastatic lesions by immunofluorescence. (A) Double staining of primary BrCa and BrCa metastatic tumors in the brain specimens for stem cell markers CD44 (green membrane staining) and CD24 (red membrane staining) reveals 2 distinct populations of cancer cells (red and green) with no detectable signal colocalization (yellow). (B, C) A 4-color immunostaining reveals loss of KISS1 expression in the BrCa stem cell population of brain metastases. (B) A representative (merged) image (400X, scale bar: 20 µm) showing colocalization of KISS1 with CD44 (magenta signals), but not with CD24 in primary BrCa specimen (RONC1), suggesting that KISS1 is expressed in primary BrCa cells or CSC; KISS1 pseudo-colored with red; CD24 with green; CD44 with blue; and DAPI with gray. (C) Representative (merged) images (400X, scale bar: 20 µm) of brain metastatic tumor (RONC-BM and RONC-BM35) specimens, showing colocalization of CD44 with FLOT2 (sky blue signal indicated by arrow), but not KISS1, in contrast to primary (RONC1) tumors, where CD44 and FLOT2 colocalize with KISS1 resulting in numerous white foci (dashed arrow), not present in metastases; CD44 (blue membrane staining), KISS1 (red cytoplasmic and nuclear staining), DAPI (gray nuclear staining), FLOT2 (green cytoplasmic and membrane staining); Insets (white boxes) show portions of the corresponding images of primary (RONC1, panel B) or metastatic (RONC-BM and RONC-BM35, panel C) tumor specimens at higher magnification. (D) Quantitative assessment of CSC with no KISS1 expression (CD44+, FLOT2+ and KISS1−, sky blue) and KISS1-positive (CD44+, FLOT2+ andKISS1+, white foci) CSC in primary and the brain metastatic specimens (white and black bars, respectively). The amount of KISS1-positive and negative CSC was determined in unmatched specimens (primary BrCa, N = 4) and BrCa metastases to the brain (N = 4) by counting sky-blue and white foci of IF signals on merged images. Each tumor type was analyzed in triplicates with 5 high power fields (HPF) ± SD (standard deviation) and minimum 50 cells per slide.

Article Snippet: The following primary antibodies were used for western blotting: rabbit anti-ATG5 (Cell Signaling Technology, 2630), anti-ATG7 (Cell Signaling Technology, 2613), anti-SQSTM1/p62 (Abcam, EPR4844), anti-LC3A/B (Novus Bio, NB100–233), anti-MMP14 (Abcam, AB38971), anti-MMP9 (Cell Signaling Technology, 2270) mouse anti-hexon (EMD Millipore, MAB8044), anti-GAPDH (EMD Millipore, MAB374); rabbit; for immunofluorescence: rabbit anti-KISS1 (Sigma-Aldrich, K0394), anti-BCL2 (Epitomics-Abcam, AP1303a), anti-CD24 (LSBio, c41096), anti-CD44 (Novus Bio, NBP1–41266) anti-CCL2 (GeneTex, GTX81767) anti-CXCL12 (LSbio, LS-c167170) and mouse FITC-labeled anti-FLOT2/ESA1 (GeneTex, GTX33201), anti-GFAP (Molecular Probes, {"type":"entrez-nucleotide","attrs":{"text":"A21282","term_id":"514150","term_text":"A21282"}} A21282 ); and histology: rabbit anti-MMP9 (Novus Bio, NB110–57222) and mouse anti-KISS1 (clone 6a4.27) provided by D. R. Welch (KUMC).

Techniques: Immunofluorescence, Double Staining, Staining, Immunostaining, Expressing, Standard Deviation